Issue 61, 2017

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

Abstract

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

Graphical abstract: Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

Supplementary files

Article information

Article type
Communication
Submitted
29 Jun 2017
Accepted
07 Jul 2017
First published
07 Jul 2017

Chem. Commun., 2017,53, 8541-8544

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

Q. Wu, B. A. Fenton, J. L. Wojtaszek and P. Zhou, Chem. Commun., 2017, 53, 8541 DOI: 10.1039/C7CC05021F

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