Issue 39, 2017
From the journal:

Chemical Communications

The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells†

Pol Sanllehí,ab   Mireia Casasampere,ab   José-Luis Abad, ORCID logo a   Gemma Fabriàs, ORCID logo a   Olga López,c   Jordi Bujons,d   Josefina Casas ORCID logo *a  and  Antonio Delgado ORCID logo *ab  

* Corresponding authors

a Spanish National Research Council (CSIC), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Research Unit on Bioactive Molecules (RUBAM), Department of Biomedicinal Chemistry, Jordi Girona 18 26, 08034 Barcelona, Spain
E-mail: fina.casas@iqac.csic.es, delgado@rubam.net

b University of Barcelona (UB), Faculty of Pharmacy and Food Sciences, Department of Pharmacology, Toxicology and Medicinal Chemistry, Unit of Pharmaceutical Chemistry (Associated Unit to CSIC), Avda. Joan XXIII s/n, 08028 Barcelona, Spain

c Spanish National Research Council (CSIC), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Group of Biophysics of Lipids and Interfaces (BLI), Department of Chemical and Surfactants Technology, Jordi Girona 18 26, 08034 Barcelona, Spain

d Spanish National Research Council (CSIC), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Department of Biological Chemistry and Molecular Modelling, Jordi Girona 18-26, 08034-Barcelona, Spain

Abstract

New fluorogenic sensors with suitable kinetic parameters and sensitivity have been developed for the determination of sphingosine-1-phosphate lyase activity in cell lysates. The probe RBM148 can be efficiently loaded into cationic liposomes and used to determine S1PL activity in intact cells.

Graphical abstract: The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells

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Article information

DOI
https://doi.org/10.1039/C7CC01659J
Article type
Communication
Submitted
03 Mar 2017
Accepted
23 Apr 2017
First published
24 Apr 2017

Chem. Commun., 2017,53, 5441-5444

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The first fluorogenic sensor for sphingosine-1-phosphate lyase activity in intact cells

P. Sanllehí, M. Casasampere, J. Abad, G. Fabriàs, O. López, J. Bujons, J. Casas and A. Delgado, Chem. Commun., 2017, 53, 5441 DOI: 10.1039/C7CC01659J

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