Pico-HPLC system integrating an equal inner diameter femtopipette into a 900 nm I.D. porous layer open tubular column†
A picoflow high performance liquid chromatography (pico-HPLC) system was developed, which could directly pipette femtoliter samples using a separation column tip driven by an electroosmotic pump. Amino acid enantiomers were separated in the 900 nm I.D. porous layer open tubular column at a flow rate of 13.50 pL min−1.