Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 8, 2017
Previous Article Next Article

Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis

Author affiliations

Abstract

A novel sample preparation method was established for proteomic analysis, during which living cells were absorbed into vacuum-dried polyacrylamide gel and directly digested into peptides for subsequent LC-MS/MS assays. As a consequence, both of the steps for cell lysis and protein extraction involved in a conventional digestion method were skipped.

Graphical abstract: Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis

Back to tab navigation

Supplementary files

Article information


Submitted
15 Oct 2016
Accepted
22 Dec 2016
First published
22 Dec 2016

Chem. Commun., 2017,53, 1421-1424
Article type
Communication

Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis

Y. Xiong, Y. Zhang, J. Yao, G. Yan and H. Lu, Chem. Commun., 2017, 53, 1421
DOI: 10.1039/C6CC08316A

Social activity

Search articles by author

Spotlight

Advertisements