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Issue 1, 2017
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A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays†

Shanshan Li,a   Li Zhou,b   Yongpeng Yao,a   Keqiang Fan, ORCID logo a   Zilong Li,a   Lixin Zhang,ab   Weishan Wang*a  and  Keqian Yang*a  
Author affiliations

* Corresponding authors

a State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China
E-mail: yangkq@im.ac.cn, wangws@im.ac.cn

b Institute of Health Sciences, Anhui University, Hefei, China

Abstract

A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors.

Graphical abstract: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

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Article information

https://doi.org/10.1039/C6CC07244E
Submitted
05 Sep 2016
Accepted
15 Nov 2016
First published
15 Nov 2016
Chem. Commun., 2017,53, 99-102
Article type
Communication
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A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

S. Li, L. Zhou, Y. Yao, K. Fan, Z. Li, L. Zhang, W. Wang and K. Yang, Chem. Commun., 2017, 53, 99
DOI: 10.1039/C6CC07244E

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