Facile fabrication of an “off–on” photoelectrochemical aptasensor for kanamycin detection based on polypyrrole/CeO2

Abstract

In this work, an “off–on” photoelectrochemical sensing strategy for the determination of trace kanamycin is proposed by using a kanamycin aptamer as the sensing unit, gold nanoparticles (AuNPs) and polypyrrole as the signal amplification unit and cerium dioxide as the photoelectric active material. The proposed method has a good linear range from 0.5–200 μg L⁻¹ with a detection limit of 0.2 μg L⁻¹ (S/N = 3). The present aptasensor showed excellent specificity and high sensitivity when it was used to determine the amount of kanamycin in milk and kanamycin sulfate injection.