A gold nanoparticles growth-based immunoassay for detection of antibiotic residues

Abstract

A quantitative immunosensor for determination of cloxacillin in foodstuffs was developed by combining glucose oxidase controlled growth of gold nanoparticles (AuNPs) with an indirect competitive enzyme-linked immunosorbent assay. The AuNPs growth-based approach allowed quick visualization of the results with the naked eye when the concentration of cloxacillin exceeded 11 ng mL⁻¹.