Issue 5, 2017

A new turn-on fluorescent probe towards hypochlorite in living cells

Abstract

A novel turn-on fluorescent sensor (PIPH) based on phenanthroimidazole derivatives was successfully designed and synthesized. The probe shows high selectivity and sensitivity towards OCl with approximately 20-fold fluorescence enhancement at 415 nm and the detection limit was measured to be 0.58 μM. Then through NMR spectra and high-resolution mass spectrometry, it was confirmed that the phenylhydrazine group of PIPH was decomposed by OCl. The fluorescence enhancement mechanism of the probe was further investigated by theoretical calculations. Finally, Hela cells were applied in cell imaging. It was proved that the probe has potential capacity to detect hypochlorite in vivo.

Graphical abstract: A new turn-on fluorescent probe towards hypochlorite in living cells

Supplementary files

Article information

Article type
Paper
Submitted
14 Oct 2016
Accepted
10 Jan 2017
First published
11 Jan 2017

Anal. Methods, 2017,9, 864-870

A new turn-on fluorescent probe towards hypochlorite in living cells

J. Guo, Z. Zhang, Z. Kuai, R. Wang, Q. Yang, Y. Shan and Y. Li, Anal. Methods, 2017, 9, 864 DOI: 10.1039/C6AY02819E

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