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Issue 21, 2016
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Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

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Abstract

A novel amperometric biosensor for glucose detection was fabricated based on a mimetic multi-enzyme system by combing mimetic enzymes (metal–organic frameworks, MOFs) and glucose oxidase (GOx). The microcapsule constructed with polydopamine (PDA) and ZIF-8 using CaCO3 templates could be utilized not only to provide the biocompatible and non-toxic microenvironment to immobilize GOx, but also as an efficient mimetic enzyme because of the peroxidase-like properties of ZIF-8. The graphene nano-sheets (rGO) were bonded to the PDA/ZIF-8 microcapsules to accelerate the electron transfer between the microcapsule and the electrode, and the resulted PDA/ZIF-8@rGO showed excellent electro-catalytic activity towards H2O2. The mimetic multi-enzyme system was fabricated when GOx was immobilized in the as-prepared microcapsule, and the developed GOx/PDA/ZIF-8@rGO/GCE biosensor exhibited extraordinary electro-detection performance for glucose with two wide linear ranges from 1 μM to 1.2 mM and 1.2 mM to 3.6 mM, and the limit of detection was 0.333 μM (S/N = 3). Moreover, the good selectivity for glucose and satisfactory detection results in real samples of the prepared biosensor were also demonstrated.

Graphical abstract: Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

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Publication details

The article was received on 31 Jan 2016, accepted on 15 Apr 2016 and first published on 18 Apr 2016


Article type: Paper
DOI: 10.1039/C6TB00276E
Citation: J. Mater. Chem. B, 2016,4, 3695-3702
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    Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

    Y. Wang, C. Hou, Y. Zhang, F. He, M. Liu and X. Li, J. Mater. Chem. B, 2016, 4, 3695
    DOI: 10.1039/C6TB00276E

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