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Issue 9, 2016
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Recyclable Cu(I)/melanin dots for cycloaddition, bioconjugation and cell labelling

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Abstract

Development of biocompatible and high-performance heterogeneous catalysts for bioconjugation and cell labeling is highly challenging. Melanin has previously been used as a target for melanoma imaging and therapy. Herein, this important biomarker was transferred into a novel catalytic platform. A biocompatible Cu(I)/melanin dot-based catalyst [Cu(I)/M-dots] was easily prepared and exhibited high catalytic activity and excellent reusability in various Cu(I)-catalyzed azide-alkyne cycloadditions (CuAAC). Furthermore, DNA bioconjugation was carried out efficiently using Cu(I)/M-dots under ligand-free and reductant-free conditions, and the Cu(I)/M-dots could easily be removed by centrifugation. Lastly, the integrin receptor (alkyne RGD targeted) of U87MG cells was effectively labelled with a fluorescent dye (Cyanine5.5 azide) in combination with Cu(I)/M-dots. These attractive properties of Cu(I)/M-dots render it a promising catalytic platform in bioconjugation and chemical biology applications.

Graphical abstract: Recyclable Cu(i)/melanin dots for cycloaddition, bioconjugation and cell labelling

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Publication details

The article was received on 07 Apr 2016, accepted on 18 May 2016 and first published on 20 May 2016


Article type: Edge Article
DOI: 10.1039/C6SC01536K
Citation: Chem. Sci., 2016,7, 5888-5892
  • Open access: Creative Commons BY license
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    Recyclable Cu(I)/melanin dots for cycloaddition, bioconjugation and cell labelling

    Y. Sun, S. Hong, X. Ma, K. Cheng, J. Wang, Z. Zhang, M. Yang, Y. Jiang, X. Hong and Z. Cheng, Chem. Sci., 2016, 7, 5888
    DOI: 10.1039/C6SC01536K

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