Jump to main content
Jump to site search

Issue 8, 2016
Previous Article Next Article

A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

Author affiliations

Abstract

Herein, we present the rational design, synthesis, characterization, and biological applications of a new rhodamine-based fluorescent probe, HKYellow, for the detection and molecular imaging of peroxynitrite, an important highly reactive oxidant involved in a variety of physiological and pathological processes. HKYellow was rationally designed on the basis of the efficient fluorescence quenching effect of the N-phenyl group to the rhodamine core and a peroxynitrite-triggered N-dearylation reaction to achieve a sensitive and selective fluorescence turn-on response toward peroxynitrite in chemical and biological levels. This probe has been thoroughly evaluated for the robust imaging of peroxynitrite in live cells and tissues. By utilizing HKYellow, we provide the first visual evidence that peroxynitrite is generated in mouse liver tissues under an acute alcohol binge or ischemic–reperfusion condition. This probe should be a powerful molecular imaging tool for interrogating the complex biological roles of peroxynitrite under various biological settings.

Graphical abstract: A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Jan 2016, accepted on 22 Apr 2016 and first published on 26 Apr 2016


Article type: Edge Article
DOI: 10.1039/C6SC00012F
Citation: Chem. Sci., 2016,7, 5407-5413
  • Open access: Creative Commons BY license
  •   Request permissions

    A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues

    T. Peng, X. Chen, L. Gao, T. Zhang, W. Wang, J. Shen and D. Yang, Chem. Sci., 2016, 7, 5407
    DOI: 10.1039/C6SC00012F

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements