Jump to main content
Jump to site search

Issue 11, 2017
Previous Article Next Article

Preferred cell alignment along concave microgrooves

Author affiliations

Abstract

Geometrical cues in the extracellular environment are essential for guiding the direction of cells and tissue architectures. For example, nano/micro-scale topography such as grooves and fibrous scaffolds induces cell alignment and migration through contact guidance. However, previous research has focused on patterns with scales ranging from nanometers to several microns. It remains unclear how cell behavior is affected by geometric cues at larger scales, such as the cylindrical curvature in the interior surface of blood vessels. Here, using microfabricated concave microgrooves to culture vascular endothelial cells, we examine how the radius of curvature affects cell body characteristics including shape, spreading area, and preferred alignment along the microgroove direction. Furthermore, analysis of subcellular actin filaments reveals that subcellular stress fibers play an essential role in such morphological response. Together, our findings not only broaden the knowledge basis of surface curvature as a biophysical factor but also offer cell characterization and contact guidance strategies for future cell and tissue engineering applications.

Graphical abstract: Preferred cell alignment along concave microgrooves

Back to tab navigation

Article information


Submitted
09 Nov 2016
Accepted
12 Jan 2017
First published
20 Jan 2017

This article is Open Access

RSC Adv., 2017,7, 6788-6794
Article type
Communication

Preferred cell alignment along concave microgrooves

B. Sun, K. Xie, T. Chen and R. H. W. Lam, RSC Adv., 2017, 7, 6788
DOI: 10.1039/C6RA26545F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements