Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H2S

Gunwoo Kim; Eunju Jang; Alexis M. Page; Ting Ding; Kimberly A. Carlson; Haishi Cao

doi:10.1039/C6RA20478C

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Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H₂S†

Gunwoo Kim,^a Eunju Jang,^a Alexis M. Page,^b Ting Ding,^a Kimberly A. Carlson

^b and Haishi Cao*^a

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* Corresponding authors

^a Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, USA 68849
E-mail: caoh1@unk.edu

^b Department of Biology, University of Nebraska-Kearney, Kearney, NE, USA 68849

Abstract

A new reaction-based sensor (AHS) was synthesized for quantitative detection of H₂S. AHS showed a high selectivity and sensitivity toward H₂S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H₂S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H₂S level, which were used to measure concentration of H₂S in the range of 0–100 μM.

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Article information

DOI: https://doi.org/10.1039/C6RA20478C
Article type: Paper
Submitted: 13 Aug 2016
Accepted: 03 Oct 2016
First published: 03 Oct 2016

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RSC Adv., 2016,6, 95920-95924

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Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H₂S

G. Kim, E. Jang, A. M. Page, T. Ding, K. A. Carlson and H. Cao, RSC Adv., 2016, 6, 95920 DOI: 10.1039/C6RA20478C

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