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Issue 102, 2016, Issue in Progress
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Borage oil supplementation decreases lipopolysaccharide-induced inflammation and skeletal muscle wasting in mice

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Abstract

Because in vitro data have shown gamma-linolenic acid (GLA) to be protective in LPS-induced macrophage inflammation and myotube atrophy, we explored the therapeutic value of borage oil (BO), a GLA rich oil, in LPS-induced inflammation and muscle wasting in C57BL/6JNarl mice. Supplementation with BO was more potent than supplementation with soybean oil (SO) in decreasing LPS-induced expression of pro-inflammatory cytokines and glutathione in serum and tissues. Notably, GLA did not reverse LPS-induced inflammatory cytokine expression in C2C12 myotubes transfected with a constitutively active mutant IκB kinase-β plasmid, which suggested the importance of the inhibition of nuclear factor-κB (NF-κB) activation by GLA. Moreover, BO prevented LPS-induced skeletal muscle weight loss as well as molecule expression of ubiquitin-proteasome pathway and the autophagy-lysosomal pathway which played a key role in skeletal muscle protein degradation. BO but not SO reduced the LPS-induced increase in toll-like receptor 4 (TLR4) expression and activation of mitogen-activated protein kinases (MAPKs) and NF-κB in gastrocnemius muscle. In summary, supplementation with BO is more effective than supplementation with SO in preventing LPS-induced inflammation and muscle wasting. Blockade of the TLR4/MAPKs/NF-κB pathway is crucial in the action of BO on LPS-induced inflammation and wasting in skeletal muscle.

Graphical abstract: Borage oil supplementation decreases lipopolysaccharide-induced inflammation and skeletal muscle wasting in mice

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Article information


Submitted
31 May 2016
Accepted
11 Oct 2016
First published
14 Oct 2016

RSC Adv., 2016,6, 100174-100185
Article type
Paper

Borage oil supplementation decreases lipopolysaccharide-induced inflammation and skeletal muscle wasting in mice

P. Chen, C. Liu, L. Lin, Y. Lin, H. Sun, C. Li, H. Chen, T. Wang, J. Wang and K. Liu, RSC Adv., 2016, 6, 100174
DOI: 10.1039/C6RA14163C

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