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Issue 81, 2016
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The role of a xylose isomerase pathway in the conversion of xylose to lipid in Mucor circinelloides

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Abstract

The xylose isomerase (XI) pathway, which converts xylose in lignocellulosic materials into intermediate metabolites, does not commonly exist in filamentous fungi. The genomic sequence of Mucor circinelloides shows that it has the XI pathway but it has never been characterized. In this study, the genes coding for XI and xylulokinase (XK) were overexpressed in Mucor circinelloides to examine their effects on xylose utilization and the xylose metabolism pathway. The results showed that overexpressing XI or XK increased the total consumption rate of xylose and the lipid yield. In the lipid accumulation phase, the mRNA levels of XI and XK, and activities of XI, XK, glucose 6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in recombinant strains were higher than the control. Our study suggested that the XI pathway plays an important role in xylose utilization and lipid accumulation in the filamentous fungus Mucor circinelloides.

Graphical abstract: The role of a xylose isomerase pathway in the conversion of xylose to lipid in Mucor circinelloides

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Article information


Submitted
12 May 2016
Accepted
05 Aug 2016
First published
05 Aug 2016

RSC Adv., 2016,6, 77944-77952
Article type
Paper

The role of a xylose isomerase pathway in the conversion of xylose to lipid in Mucor circinelloides

L. Chu, X. Zan, X. Tang, L. Zhao, H. Chen, Y. Q. Chen, W. Chen and Y. Song, RSC Adv., 2016, 6, 77944
DOI: 10.1039/C6RA12379A

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