Fabrication of a novel chitosan scaffold with asymmetric structure for guided tissue regeneration

Abstract

A novel chitosan (CS)-based asymmetric scaffold composed of a dense layer and a loose layer was fabricated via the self-deposition approach. The micro-structure and mechanical properties were verified to satisfy the criteria for the guided tissue regeneration (GTR) membrane. The in vitro biocompatibility of the scaffold was verified by the CCK-8 assays of MC3T3 osteoblastic cells. The rat skull defect model was applied to estimate the in vivo bone regeneration performance. The micro-CT and histological results manifested its outstanding bone regenerative ability and superior degradability over the Bio-gide® membrane. Considering the relative low expense and simple fabrication process, the prepared asymmetric CS scaffold is a prospective candidate for GTR therapy.