Layer-by-layer assembly of hemoglobin-coated microspheres for enhancing the oxygen carrying capacity

Abstract

Hemoglobin-coated microspheres with one layer and five layers were fabricated by layer-by-layer assembly. The electrochemical methods were used to study their capacity for carrying oxygen. The results provided direct evidences that microspheres with five-layer hemoglobin could store and release more oxygen than those with one layer. This study also provided an alternative method for evaluating the oxygen carrying capacity of hemoglobin microspheres.