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Issue 24, 2016
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β-Cyclodextrin included coumarin derivatives as selective fluorescent sensors for Cu2+ ions in HeLa cells

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Abstract

A highly sensitive and selective fluorescent sensor for Cu2+ ions in water medium is reported using 4-((benzo[d]thiazol-2-ylthio)methyl)-5,7-dihydroxy-2H-chromen-2-one (1) included β-cyclodextrin, as a probe. The fluorogenic supramolecule has displayed good selectivity and affinity towards Cu2+ ions over other cations after examining in biological systems with intracellular Cu2+ ions, especially in cultured HeLa cells using fluorescence microscopic imaging. The lowest detection limit of Cu2+ ions observed using this probe is as low as 2.52 × 10−10 M. The observed on-off fluorescence with the periodic addition of Cu2+ ion is explained via an Intramolecular Charge Transfer mechanism (ICT) and the inclusion of 1 in β-cyclodextrin is characterised by 1H-NMR molecular modeling studies. The results show that the present β-CD:1 system, studied in HeLa cells, can be potentially used in monitoring the biological functions of Cu2+ ions.

Graphical abstract: β-Cyclodextrin included coumarin derivatives as selective fluorescent sensors for Cu2+ ions in HeLa cells

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Supplementary files

Article information


Submitted
18 Jan 2016
Accepted
11 Feb 2016
First published
12 Feb 2016

RSC Adv., 2016,6, 20269-20275
Article type
Paper

β-Cyclodextrin included coumarin derivatives as selective fluorescent sensors for Cu2+ ions in HeLa cells

R. I. Khan and K. Pitchumani, RSC Adv., 2016, 6, 20269
DOI: 10.1039/C6RA01522K

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