Issue 38, 2016

A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells

Abstract

A ratiometric fluorescent probe with strong intramolecular charge transfer (ICT) character has been designed for the detection of alkaline phosphatase (ALP). In the presence of ALP, probe 1 showed a dramatic bathochromic shift from 550 to 650 nm in the fluorescence spectrum and an obvious ratiometric signal with an isoemissive point was observed. The spectral change was attributed to the hydrolysis and cleavage of a phosphate group from the probe that changed the ability of the intramolecular charge transfer. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 50 to 200 U L−1. Other biological species, including lysozyme, trypsin, pepsin, acetyl cholinesterase, carboxylesterase, and bovine serum albumin, did not induce distinct spectral changes of the probe, indicating its selective sensing ability to ALP. Furthermore, probe 1 was also successfully applied to image endogenous ALP activity in living cells.

Graphical abstract: A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells

Supplementary files

Article information

Article type
Paper
Submitted
12 Jan 2016
Accepted
21 Mar 2016
First published
24 Mar 2016

RSC Adv., 2016,6, 32046-32051

A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells

Z. Lu, J. Wu, W. Liu, G. Zhang and P. Wang, RSC Adv., 2016, 6, 32046 DOI: 10.1039/C6RA00983B

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