Issue 21, 2016

Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: application to pharmaceutical dosage forms

Abstract

A novel capillary electrophoretic method was developed for the assay of two quaternary anti-Parkinson mixtures, entacapone, levodopa, carbidopa, and benserazide (mixture I), and selegiline, levodopa, carbidopa, and benserazide (mixture II), by using α-methyldopa as an internal standard. Furthermore, the method was extended for the determination of another anti-Parkinson drug, lisuride, as well as a psychoactive antihypertensive drug, α-methyldopa, without any modification of the general method. Separation and analyses of all compounds were simply achieved in an untreated fused-silica capillary tube (42.0 cm effective length and 50 μm internal diameter) within 7 minutes under an applied voltage of 20 kV. Optimum separation and analyses were obtained using 25 mM borate buffer (pH 9.5) containing 5 mM β-cyclodextrin as the background electrolyte. The apparatus was equipped with a diode array detector (DAD) to identify lisuride at 240 nm and all other drugs at 200 nm. The addition of 5 mM β-cyclodextrin to the borate buffer has a significant effect on the separation of entacapone and benserazide in mixture I, and on the separation of selegiline and benserazide in mixture II, which cannot be achieved without it. The proposed method was successfully applied to analyse the studied drugs in their multi-component and single-component pharmaceutical dosage forms. The analytical results proved the linearity (r2 ≥ 0.9997), accuracy, precision (% RSD < 2), and selectivity of the proposed capillary electrophoretic method.

Graphical abstract: Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: application to pharmaceutical dosage forms

Article information

Article type
Paper
Submitted
11 Dec 2015
Accepted
26 Jan 2016
First published
01 Feb 2016

RSC Adv., 2016,6, 17519-17530

Author version available

Determination of six anti-Parkinson drugs using cyclodextrin-capillary electrophoresis method: application to pharmaceutical dosage forms

A. M. Zeid, J. J. M. Nasr, F. F. Belal, S. Kitagawa, N. Kaji, Y. Baba and M. I. Walash, RSC Adv., 2016, 6, 17519 DOI: 10.1039/C5RA26473A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements