Analysis of sphingomyelin in plasma membrane at single cells using luminol electrochemiluminescence

Abstract

Sphingomyelin in plasma membrane at single cells was analyzed using luminol electrochemiluminescence, which involved serial reactions to generate hydrogen peroxide from membrane sphingomyelin for luminescence detection. A large deviation of the luminescence ratio before and after the introduction of the enzyme mixture was obtained from single cell analysis indicating the high cellular heterogeneity.