A novel aptamer-mediated CuInS2 quantum dots@graphene oxide nanocomposites-based fluorescence “turn off–on” nanosensor for highly sensitive and selective detection of kanamycin

Abstract

In this paper, we designed a novel near-infrared aptamer-mediated fluorescence “turn off–on” nanosensor for highly sensitive and selective detection of kanamycin based on CuInS₂ quantum dots (QDs)@graphene oxide (GO) nanocomposites. The carboxy groups on the surface of CuInS₂ QDs (modified with mercaptopropionic acid) were conjugated with amino terminal kanamycin-binding Ky2 aptamer to form the Ky2-CuInS₂ QDs in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. Then, the Ky2-CuInS₂ QDs were facilely immobilized on the surface of GO through π–π stacking interaction between the nucleobases and GO, which caused the fluorescence of Ky2-CuInS₂ QDs “turned off”. In the presence of kanamycin, the Ky2-CuInS₂ QDs desorb from the surface of GO and bind to kanamycin with high affinity and specificity. As a result, the quenched fluorescence “turned on”. Under the optimum conditions, there was a good linear relationship between I/I₀ (I and I₀ were the fluorescence intensity of Ky2-CuInS₂ QDs@GO in the presence and absence of kanamycin, respectively) and kanamycin concentration in the range of 0.3–45 nmol L⁻¹ (0.174–26.1 μg L⁻¹), with the detection limit of 0.12 nmol L⁻¹ (0.070 μg L⁻¹). The present nanosensor was utilized to detect kanamycin in the human serum, urine and milk samples with satisfactory results.