Jump to main content
Jump to site search

Issue 11, 2016
Previous Article Next Article

Towards efficient biocatalysts: photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils

Author affiliations

Abstract

Herein, we report the preparation and characterization of a new biocatalyst based on the photo-immobilization of a lipase onto hybrid protein : sugar nanofibrils. The nanofibrils are obtained through the aggregation of hen white egg lysozyme induced by the highly sulfated glycosaminoglycan heparin. The new hybrid nanomaterial could be easily functionalized using photochemical reaction to attach lipases through dityrosine covalent bonds. Compared to the free enzyme, the photo-immobilized lipase has better thermostability and enhanced resistance to non-conventional environment. Structural and morphological characterization of the nanofibrils shows that they are compatible with amyloid-like aggregates. In addition, the supramolecular arrangement of heparin and lysozyme within the building unit of the nanofibrils is also proposed. The procedure reported herein could be useful to design a new generation of insoluble biocatalyst by a single photo-click step which is definitely cleaner and faster than conventional chemical cross-linked procedures.

Graphical abstract: Towards efficient biocatalysts: photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Sep 2015, accepted on 07 Jan 2016 and first published on 11 Jan 2016


Article type: Paper
DOI: 10.1039/C5RA19590J
Author version
available:
Download author version (PDF)
RSC Adv., 2016,6, 8528-8538

  •   Request permissions

    Towards efficient biocatalysts: photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils

    S. Chaves, L. M. Pera, C. L. Avila, C. M. Romero, M. Baigori, F. E. Morán Vieyra, C. D. Borsarelli and R. N. Chehin, RSC Adv., 2016, 6, 8528
    DOI: 10.1039/C5RA19590J

Search articles by author

Spotlight

Advertisements