Jump to main content
Jump to site search

Issue 26, 2016
Previous Article Next Article

Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

Author affiliations

Abstract

SalL, an enzyme that catalyzes the synthesis of SAM from L-methionine and 5′-chloro-5′-deoxyoadenosine, is shown to accept 5′-chloro-5′-deoxythienoadenosine as a substrate and facilitate the synthesis of a synthetic SAM analog with an unnatural nucleobase. This synthetic cofactor is demonstrated to replace SAM in the DNA methylation reaction with M.TaqI.

Graphical abstract: Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

Back to tab navigation

Supplementary files

Article information


Submitted
19 Apr 2016
Accepted
24 May 2016
First published
24 May 2016

This article is Open Access

Org. Biomol. Chem., 2016,14, 6189-6192
Article type
Communication

Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

C. Vranken, A. Fin, P. Tufar, J. Hofkens, M. D. Burkart and Y. Tor, Org. Biomol. Chem., 2016, 14, 6189
DOI: 10.1039/C6OB00844E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements