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Issue 23, 2016
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Clamping of RNA with PNA enables targeting of microRNA

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Abstract

To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.

Graphical abstract: Clamping of RNA with PNA enables targeting of microRNA

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Publication details

The article was received on 07 Mar 2016, accepted on 12 May 2016 and first published on 16 May 2016


Article type: Communication
DOI: 10.1039/C6OB00516K
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Citation: Org. Biomol. Chem., 2016,14, 5210-5213
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    Clamping of RNA with PNA enables targeting of microRNA

    A. Ghidini, H. Bergquist, M. Murtola, T. Punga, R. Zain and R. Strömberg, Org. Biomol. Chem., 2016, 14, 5210
    DOI: 10.1039/C6OB00516K

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