Issue 7, 2016
From the journal:

Organic & Biomolecular Chemistry

Simple and rapid quantification of phospholipids for supramolecular membrane transport assays†

Robert Hein,a   Can B. Uzundal‡a  and  Andreas Hennig*a  

* Corresponding authors

a Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
E-mail: a.hennig@jacobs-university.de

Abstract

We introduce a simple 1H NMR method for quantification of the phospholipid content of liposomes. The method is validated by comparison with the established Stewart assay, which revealed significant uncertainties in phospholipid quantification of established liposome preparations used in supramolecular membrane transport assays.

Graphical abstract: Simple and rapid quantification of phospholipids for supramolecular membrane transport assays

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Article information

DOI
https://doi.org/10.1039/C5OB02480C
Article type
Communication
Submitted
04 Dec 2015
Accepted
15 Jan 2016
First published
15 Jan 2016
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2016,14, 2182-2185

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Simple and rapid quantification of phospholipids for supramolecular membrane transport assays

R. Hein, C. B. Uzundal and A. Hennig, Org. Biomol. Chem., 2016, 14, 2182 DOI: 10.1039/C5OB02480C

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