Issue 3, 2016
From the journal:

Organic & Biomolecular Chemistry

Semisynthetic UbH2A reveals different activities of deubiquitinases and inhibitory effects of H2A K119 ubiquitination on H3K36 methylation in mononucleosomes†

Xiaobao Bi,‡a   Renliang Yang,‡a   Xiaoyu Feng,a   Daniela Rhodesa  and  Chuan-Fa Liu*a  

* Corresponding authors

a Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
E-mail: cfliu@ntu.edu.sg
Fax: +65 67953021

Abstract

Using a genetically incorporated azidonorleucine for ubiquitin installation, we prepared multi-milligram quantities of H2AK119ub (ubH2A). With a native isopeptide linkage, the synthetic ubH2A was used to study the activity of deubiquitinases and crosstalk between H2A ubiquitination and H3K36 methylation in the context of chemically defined mononucleosomes.

Graphical abstract: Semisynthetic UbH2A reveals different activities of deubiquitinases and inhibitory effects of H2A K119 ubiquitination on H3K36 methylation in mononucleosomes

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Article information

DOI
https://doi.org/10.1039/C5OB02323H
Article type
Communication
Submitted
11 Nov 2015
Accepted
23 Nov 2015
First published
24 Nov 2015

Org. Biomol. Chem., 2016,14, 835-839

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Semisynthetic UbH2A reveals different activities of deubiquitinases and inhibitory effects of H2A K119 ubiquitination on H3K36 methylation in mononucleosomes

X. Bi, R. Yang, X. Feng, D. Rhodes and C. Liu, Org. Biomol. Chem., 2016, 14, 835 DOI: 10.1039/C5OB02323H

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