A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.
T. Merkle, I. T. Holder and J. S. Hartig, Org. Biomol. Chem., 2016, 14, 447 DOI: 10.1039/C5OB02026C
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