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Issue 48, 2016
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Single-molecule fluorescence resonance energy transfer in molecular biology

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Abstract

Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open–close properties, receptor–ligand interactions, nucleic acid structure regulation, vesicle fusion, and force induced conformational dynamics. Finally, we discuss the main limitations of smFRET in molecular biology.

Graphical abstract: Single-molecule fluorescence resonance energy transfer in molecular biology

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Publication details

The article was received on 27 Aug 2016, accepted on 14 Nov 2016 and first published on 16 Nov 2016


Article type: Review Article
DOI: 10.1039/C6NR06794H
Nanoscale, 2016,8, 19928-19944

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    Single-molecule fluorescence resonance energy transfer in molecular biology

    D. K. Sasmal, L. E. Pulido, S. Kasal and J. Huang, Nanoscale, 2016, 8, 19928
    DOI: 10.1039/C6NR06794H

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