Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 48, 2016
Previous Article Next Article

Single-molecule fluorescence resonance energy transfer in molecular biology

Author affiliations

Abstract

Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open–close properties, receptor–ligand interactions, nucleic acid structure regulation, vesicle fusion, and force induced conformational dynamics. Finally, we discuss the main limitations of smFRET in molecular biology.

Graphical abstract: Single-molecule fluorescence resonance energy transfer in molecular biology

Back to tab navigation

Article information


Submitted
27 Aug 2016
Accepted
14 Nov 2016
First published
16 Nov 2016

Nanoscale, 2016,8, 19928-19944
Article type
Review Article

Single-molecule fluorescence resonance energy transfer in molecular biology

D. K. Sasmal, L. E. Pulido, S. Kasal and J. Huang, Nanoscale, 2016, 8, 19928
DOI: 10.1039/C6NR06794H

Social activity

Search articles by author

Spotlight

Advertisements