Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.

Issue 47, 2016
Previous Article Next Article

Mito-magneto: a tool for nanoparticle mediated mitochondria isolation

Author affiliations


The field of intracellular organelle targeting using nanoparticle (NP) is mushrooming rapidly. Thus, the area of nanotechnology-enabled targeting of mitochondrion, the cellular powerhouse, for diseases characterized by mitochondrial dysfunctions such as cancer, diseases of the central nervous system, and cardiovascular diseases is also growing at a rapid pace. Optimization of a NP's ability to target the mitochondria requires quantification of the particles in this subcellular organelle and isolation of mitochondria from the cells. Conventional gradient centrifugation used in currently available methods may not be appropriate for NP containing mitochondria isolation as these particles undergo Brownian motion under centrifugal forces yielding irreproducible results. There is only one method for centrifugation-free mitochondria isolation; however, this method requires immunoprecipitation. Thus, a reliable centrifugation and immunoprecipitation free method is urgently needed to support this growing field of nanotechnology-based mitochondria targeting. Here, we report a mitochondria-targeted magnetic NP, Mito-magneto, to avoid centrifugation and immunoprecipitation methods for isolation of functional, respiration active pure mitochondria, which can be used to analyze and quantify mitochondria targeting properties of various NPs as an important tool for the growing field of “mitochondrial nanomedicine”.

Graphical abstract: Mito-magneto: a tool for nanoparticle mediated mitochondria isolation

Back to tab navigation

Supplementary files

Article information

25 Jul 2016
22 Sep 2016
First published
13 Oct 2016

Nanoscale, 2016,8, 19581-19591
Article type

Mito-magneto: a tool for nanoparticle mediated mitochondria isolation

B. Banik, B. W. Askins and S. Dhar, Nanoscale, 2016, 8, 19581
DOI: 10.1039/C6NR05882E

Social activity

Search articles by author