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Issue 39, 2016
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Folate-based single cell screening using surface enhanced Raman microimaging

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Abstract

Recent progress in nanotechnology and its application to biomedical settings have generated great advantages in dealing with early cancer diagnosis. The identification of the specific properties of cancer cells, such as the expression of particular plasma membrane molecular receptors, has become crucial in revealing the presence and in assessing the stage of development of the disease. Here we report a single cell screening approach based on Surface Enhanced Raman Scattering (SERS) microimaging. We fabricated a SERS-labelled nanovector based on the biofunctionalization of gold nanoparticles with folic acid. After treating the cells with the nanovector, we were able to distinguish three different cell populations from different cell lines (cancer HeLa and PC-3, and normal HaCaT lines), suitably chosen for their different expressions of folate binding proteins. The nanovector, indeed, binds much more efficiently on cancer cell lines than on normal ones, resulting in a higher SERS signal measured on cancer cells. These results pave the way for applications in single cell diagnostics and, potentially, in theranostics.

Graphical abstract: Folate-based single cell screening using surface enhanced Raman microimaging

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Publication details

The article was received on 24 Jun 2016, accepted on 02 Sep 2016 and first published on 05 Sep 2016


Article type: Paper
DOI: 10.1039/C6NR05057C
Citation: Nanoscale, 2016,8, 17304-17313
  • Open access: Creative Commons BY license
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    Folate-based single cell screening using surface enhanced Raman microimaging

    C. Fasolato, S. Giantulli, I. Silvestri, F. Mazzarda, Y. Toumia, F. Ripanti, F. Mura, F. Luongo, F. Costantini, F. Bordi, P. Postorino and F. Domenici, Nanoscale, 2016, 8, 17304
    DOI: 10.1039/C6NR05057C

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