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Issue 33, 2016
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Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

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Abstract

The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. We report the use of cantilever arrays for simultaneous detection of multiple exosomal surface-antigens with high sensitivity and selectivity. Exosomes from breast cancer were selectively identified by detecting over-expressed membrane-proteins CD24, CD63, and EGFR. Excellent selectivity however, was achieved when targeting the cell-surface proteoglycan, Glypican-1 at extraordinary limits (∼200 exosomes per mL, ∼0.1 pg mL−1).

Graphical abstract: Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

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Supplementary files

Article information


Submitted
29 Apr 2016
Accepted
27 Jul 2016
First published
28 Jul 2016

Nanoscale, 2016,8, 15137-15141
Article type
Communication
Author version available

Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

H. Etayash, A. R. McGee, K. Kaur and T. Thundat, Nanoscale, 2016, 8, 15137
DOI: 10.1039/C6NR03478K

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