Issue 14, 2016

High-speed detection of DNA translocation in nanopipettes


We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.

Graphical abstract: High-speed detection of DNA translocation in nanopipettes

Supplementary files

Article information

Article type
04 Dec 2015
10 Mar 2016
First published
11 Mar 2016
This article is Open Access
Creative Commons BY license

Nanoscale, 2016,8, 7604-7611

Author version available

High-speed detection of DNA translocation in nanopipettes

R. L. Fraccari, P. Ciccarella, A. Bahrami, M. Carminati, G. Ferrari and T. Albrecht, Nanoscale, 2016, 8, 7604 DOI: 10.1039/C5NR08634E

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