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Issue 14, 2016
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High-speed detection of DNA translocation in nanopipettes

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Abstract

We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.

Graphical abstract: High-speed detection of DNA translocation in nanopipettes

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Publication details

The article was received on 04 Dec 2015, accepted on 10 Mar 2016 and first published on 11 Mar 2016


Article type: Paper
DOI: 10.1039/C5NR08634E
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Citation: Nanoscale, 2016,8, 7604-7611
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    High-speed detection of DNA translocation in nanopipettes

    R. L. Fraccari, P. Ciccarella, A. Bahrami, M. Carminati, G. Ferrari and T. Albrecht, Nanoscale, 2016, 8, 7604
    DOI: 10.1039/C5NR08634E

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