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Issue 2, 2016
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Quantification of proteins using enhanced etching of Ag coated Au nanorods by the Cu2+/bicinchoninic acid pair with improved sensitivity

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Abstract

Plasmonic nanosensors show great potential in ultrasensitive detection, especially with the plasmon peak position as the detection modality. Herein, a new sensitive but simple total protein quantification method termed the SPR–BCA assay is demonstrated by combining plasmonic nanosensors with protein oxidation by Cu2+. The easy tuning of localized surface plasmon resonance (LSPR) features of plasmonic nanostructures makes them ideal sensing platforms. We found that the Cu2+/bicinchoninic acid (BCA) pair exhibits accelerated etching of Au@Ag nanorods and results in the LSPR peak shift. A linear relationship between Cu2+ and the LSPR shift is found in a double logarithmic coordinate. Such double logarithm relationship is transferred to the concentration of proteins. Theoretical simulation shows that Au nanorods with large aspect ratios and small core sizes show high detection sensitivity. Via optimized sensor design, we achieved an increased sensitivity (the limit of detection was 3.4 ng ml−1) and a wide working range (0.5 to 1000 μg ml−1) compared with the traditional BCA assay. The universal applicability of our method to various proteins further proves its potential in practical applications.

Graphical abstract: Quantification of proteins using enhanced etching of Ag coated Au nanorods by the Cu2+/bicinchoninic acid pair with improved sensitivity

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Publication details

The article was received on 10 Nov 2015, accepted on 01 Dec 2015 and first published on 07 Dec 2015


Article type: Communication
DOI: 10.1039/C5NR07924A
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Citation: Nanoscale, 2016,8, 780-784

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    Quantification of proteins using enhanced etching of Ag coated Au nanorods by the Cu2+/bicinchoninic acid pair with improved sensitivity

    W. Liu, S. Hou, J. Yan, H. Zhang, Y. Ji and X. Wu, Nanoscale, 2016, 8, 780
    DOI: 10.1039/C5NR07924A

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