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Issue 23, 2016
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High resolution atomic force microscopy of double-stranded RNA

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Double-stranded (ds) RNA mediates the suppression of specific gene expression, it is the genetic material of a number of viruses, and a key activator of the innate immune response against viral infections. The ever increasing list of roles played by dsRNA in the cell and its potential biotechnological applications over the last decade has raised an interest for the characterization of its mechanical properties and structure, and that includes approaches using Atomic Force Microscopy (AFM) and other single-molecule techniques. Recent reports have resolved the structure of dsDNA with AFM at unprecedented resolution. However, an equivalent study with dsRNA is still lacking. Here, we have visualized the double helix of dsRNA under near-physiological conditions and at sufficient resolution to resolve the A-form sub-helical pitch periodicity. We have employed different high-sensitive force-detection methods and obtained images with similar spatial resolution. Therefore, we show here that the limiting factors for high-resolution AFM imaging of soft materials in liquid medium are, rather than the imaging mode, the force between the tip and the sample and the sharpness of the tip apex.

Graphical abstract: High resolution atomic force microscopy of double-stranded RNA

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Supplementary files

Article information

26 Oct 2015
30 Jan 2016
First published
02 Feb 2016

Nanoscale, 2016,8, 11818-11826
Article type
Author version available

High resolution atomic force microscopy of double-stranded RNA

P. Ares, M. E. Fuentes-Perez, E. Herrero-Galán, J. M. Valpuesta, A. Gil, J. Gomez-Herrero and F. Moreno-Herrero, Nanoscale, 2016, 8, 11818
DOI: 10.1039/C5NR07445B

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