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Issue 14, 2016
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One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology

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Abstract

Although various types of organs-on-chips have been introduced recently as tools for drug discovery, the current studies are limited in terms of fabrication methods. The fabrication methods currently available not only need a secondary cell-seeding process and result in severe protein absorption due to the material used, but also have difficulties in providing various cell types and extracellular matrix (ECM) environments for spatial heterogeneity in the organs-on-chips. Therefore, in this research, we introduce a novel 3D bioprinting method for organ-on-a-chip applications. With our novel 3D bioprinting method, it was possible to prepare an organ-on-a-chip in a simple one-step fabrication process. Furthermore, protein absorption on the printed platform was very low, which will lead to accurate measurement of metabolism and drug sensitivity. Moreover, heterotypic cell types and biomaterials were successfully used and positioned at the desired position for various organ-on-a-chip applications, which will promote full mimicry of the natural conditions of the organs. The liver organ was selected for the evaluation of the developed method, and liver function was shown to be significantly enhanced on the liver-on-a-chip, which was prepared by 3D bioprinting. Consequently, the results demonstrate that the suggested 3D bioprinting method is easier and more versatile for production of organs-on-chips.

Graphical abstract: One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology

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Publication details

The article was received on 03 Apr 2016, accepted on 31 May 2016 and first published on 15 Jun 2016


Article type: Paper
DOI: 10.1039/C6LC00450D
Citation: Lab Chip, 2016,16, 2618-2625
  • Open access: Creative Commons BY license
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    One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology

    H. Lee and D. Cho, Lab Chip, 2016, 16, 2618
    DOI: 10.1039/C6LC00450D

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