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Issue 8, 2016
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Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

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Abstract

Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An additional strength of the technology is the possibility to perform large-scale genotypic or phenotypic screens at the single-cell level. Here we critically review the latest developments in antibody screening, drug discovery and highly multiplexed genomic applications such as targeted genetic workflows, single-cell RNAseq and single-cell ChIPseq. Starting with a comprehensive introduction for non-experts, we pinpoint current limitations, analyze how they might be overcome and give an outlook on exciting future applications.

Graphical abstract: Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

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Article information


Submitted
23 Feb 2016
Accepted
18 Mar 2016
First published
18 Mar 2016

This article is Open Access

Lab Chip, 2016,16, 1314-1331
Article type
Critical Review

Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

N. Shembekar, C. Chaipan, R. Utharala and C. A. Merten, Lab Chip, 2016, 16, 1314
DOI: 10.1039/C6LC00249H

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    [Original citation] - Published by The Royal Society of Chemistry.

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