Jump to main content
Jump to site search

Issue 4, 2016
Previous Article Next Article

Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets

Author affiliations

Abstract

Position-dependent gene expression in gradients of morphogens is one of the key processes involved in cellular differentiation during development. Here, we study a simple artificial differentiation process, which is based on the diffusion of genetic inducers within one-dimensional arrangements of 50 μm large water-in-oil droplets. The droplets are filled with either bacteria or cell-free gene expression systems, both equipped with genetic constructs that produce inducers or respond to them via expression of a fluorescent protein. We quantitatively study the coupled diffusion-gene expression process and demonstrate that gene expression can be made position-dependent both within bacteria-containing and cell-free droplets. By generating diffusing quorum sensing signals in situ, we also establish communication between artificial cell-free sender cells and bacterial receivers, and vice versa.

Graphical abstract: Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets

Back to tab navigation

Supplementary files

Publication details

The article was received on 20 Nov 2015, accepted on 11 Jan 2016 and first published on 12 Jan 2016


Article type: Paper
DOI: 10.1039/C5IB00301F
Citation: Integr. Biol., 2016,8, 564-570
  • Open access: Creative Commons BY-NC license

Search articles by author

Spotlight

Advertisements