Issue 7, 2016

An efficient enzymatic production of N-acetyl-d-glucosamine from crude chitin powders

Abstract

In this study, we present an efficient affinity adsorption–enzymatic reaction integrated approach to produce N-acetyl-D-glucosamine (GlcNAc) from crude chitin powders (CP). Chitinase was first purified from the crude enzyme mixture by affinity adsorption on the CP substrate, with ∼92.7% recovery of the enzyme achieved within 10 minutes in the presence of 20 g L−1 of CP under optimized conditions. The chitinase then efficiently converted 40 g L−1 of CP to 39.3 g L−1 of GlcNAc in a batch enzymatic reaction for 4 days, achieving nearly 100% conversion of the substrate CP. The product GlcNAc was easily separated from hydrolysates after destaining, concentrating, crystallizing and drying processes with a high purity of 98%. In addition, the chitinase can be conveniently reused for several batches by the affinity adsorption–enzymatic reaction approach, giving an average yield of 29.6 g L−1 of GlcNAc based on 30 g L−1 of CP as the substrate.

Graphical abstract: An efficient enzymatic production of N-acetyl-d-glucosamine from crude chitin powders

Supplementary files

Article information

Article type
Paper
Submitted
18 Sep 2015
Accepted
28 Nov 2015
First published
30 Nov 2015

Green Chem., 2016,18, 2147-2154

An efficient enzymatic production of N-acetyl-D-glucosamine from crude chitin powders

A. Zhang, C. Gao, J. Wang, K. Chen and P. Ouyang, Green Chem., 2016, 18, 2147 DOI: 10.1039/C5GC02242H

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