Volume 187, 2016

Assessing corneal biomechanics with Brillouin spectro-microscopy


A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.

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Article information

Article type
02 Nov 2015
07 Dec 2015
First published
06 Apr 2016
This article is Open Access
Creative Commons BY license

Faraday Discuss., 2016,187, 415-428

Assessing corneal biomechanics with Brillouin spectro-microscopy

G. Lepert, R. M. Gouveia, C. J. Connon and C. Paterson, Faraday Discuss., 2016, 187, 415 DOI: 10.1039/C5FD00152H

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