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Issue 36, 2016
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A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity

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Abstract

We describe a novel methylation-sensitive nucleic acid (RNA) probe which switches conformation according to its methylation status. When combined with a differential scanning fluorimetry technique, it enables highly sensitive and selective detection of demethylase activity at a single methylated-base level. The approach is highly versatile and may be adapted to a broad range of RNA demethylases.

Graphical abstract: A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity

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Publication details

The article was received on 02 Feb 2016, accepted on 05 Apr 2016 and first published on 07 Apr 2016


Article type: Communication
DOI: 10.1039/C6CC01045H
Citation: Chem. Commun., 2016,52, 6181-6184
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    A methylation-switchable conformational probe for the sensitive and selective detection of RNA demethylase activity

    T. Yang, A. Cheong, X. Mai, S. Zou and E. C. Y. Woon, Chem. Commun., 2016, 52, 6181
    DOI: 10.1039/C6CC01045H

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