Issue 80, 2016
From the journal:

Chemical Communications

A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates†

Jiantong Dong,a   Tongbo Wu,a   Yu Xiao,b   Lei Xu,a   Simin Fanga  and  Meiping Zhao ORCID logo *a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
E-mail: mpzhao@pku.edu.cn
Fax: +86-10-62751708

b Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

Abstract

A fuel-limited isothermal DNA machine has been built for the sensitive fluorescence detection of cellular deoxyribonucleoside triphosphates (dNTPs) at the fmol level, which greatly reduces the required sample cell number. Upon the input of the limiting target dNTP, the machine runs automatically at 37 °C without the need for higher temperature.

Graphical abstract: A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C6CC05988K
Article type
Communication
Submitted
21 Jul 2016
Accepted
05 Sep 2016
First published
07 Sep 2016

Chem. Commun., 2016,52, 11923-11926

Permissions

Request permissions

A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates

J. Dong, T. Wu, Y. Xiao, L. Xu, S. Fang and M. Zhao, Chem. Commun., 2016, 52, 11923 DOI: 10.1039/C6CC05988K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements