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Issue 55, 2016
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Genetically encoded optical activation of DNA recombination in human cells

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Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Graphical abstract: Genetically encoded optical activation of DNA recombination in human cells

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Supplementary files

Article information


Submitted
10 May 2016
Accepted
28 May 2016
First published
09 Jun 2016

This article is Open Access

Chem. Commun., 2016,52, 8529-8532
Article type
Communication

Genetically encoded optical activation of DNA recombination in human cells

J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin and A. Deiters, Chem. Commun., 2016, 52, 8529
DOI: 10.1039/C6CC03934K

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