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Issue 55, 2016
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Genetically encoded optical activation of DNA recombination in human cells

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Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Graphical abstract: Genetically encoded optical activation of DNA recombination in human cells

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Publication details

The article was received on 10 May 2016, accepted on 28 May 2016 and first published on 09 Jun 2016


Article type: Communication
DOI: 10.1039/C6CC03934K
Citation: Chem. Commun., 2016,52, 8529-8532
  • Open access: Creative Commons BY license
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    Genetically encoded optical activation of DNA recombination in human cells

    J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin and A. Deiters, Chem. Commun., 2016, 52, 8529
    DOI: 10.1039/C6CC03934K

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