Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions

Chen Xu; Jinjun Wu; Wenting Liu; Tingting Hong; Tianlu Wang; Xiaoe Zhang; Boshi Fu; Fan Wu; Zhiguo Wu; Xiang Zhou

doi:10.1039/C6CC03138B

Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions†

Chen Xu,‡^a Jinjun Wu,‡^a Wenting Liu,^a Tingting Hong,^a Tianlu Wang,^a Xiaoe Zhang,^a Boshi Fu,^a Fan Wu,^a Zhiguo Wu*^b and Xiang Zhou*^a

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* Corresponding authors

^a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University Wuhan, Hubei, P. R. China
E-mail: xzhou@whu.edu.cn

^b College of Life Science, Wuhan University Wuhan, Hubei, P. R. China
E-mail: wu.zhiguo@whu.edu.cn

Abstract

We present here a novel and efficient method for 5mC detection using a DNA strand exchange reaction (SER) strategy. This enzyme-free method needs no pre-treatment of target DNAs and can be adapted to most of the target duplexes under physiological conditions. The high sequence selectivity of this method can distinguish 5mC from normal cytosine in an accurate manner.

Chemical Communications

Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions†

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Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions

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