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Issue 53, 2016
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A click chemistry-based microRNA maturation assay optimized for high-throughput screening

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Abstract

Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels–Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.

Graphical abstract: A click chemistry-based microRNA maturation assay optimized for high-throughput screening

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Publication details

The article was received on 07 Apr 2016, accepted on 26 May 2016 and first published on 02 Jun 2016


Article type: Communication
DOI: 10.1039/C6CC02894B
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Chem. Commun., 2016,52, 8267-8270

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    A click chemistry-based microRNA maturation assay optimized for high-throughput screening

    D. A. Lorenz and A. L. Garner, Chem. Commun., 2016, 52, 8267
    DOI: 10.1039/C6CC02894B

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