Issue 47, 2016
From the journal:

Chemical Communications

Simple and sensitive microbial pathogen detection using a label-free DNA amplification assay†

Yuhuan Sun,ab   Chuanqi Zhao,*a   Zhengqing Yan,ab   Jinsong Rena  and  Xiaogang Qu*a  

* Corresponding authors

a Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
E-mail: xqu@ciac.ac.cn

b University of Chinese Academy of Sciences, Beijing 100039, China

Abstract

By the combination of quaternized magnetic nanoparticles and a label-free exonuclease III-assisted DNA amplification assay, we report a simple and facile strategy for the convenient and highly sensitive detection of microbial pathogens, with a detection limit of down to 50 cells mL−1.

Graphical abstract: Simple and sensitive microbial pathogen detection using a label-free DNA amplification assay

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Article information

DOI
https://doi.org/10.1039/C6CC02672A
Article type
Communication
Submitted
30 Mar 2016
Accepted
13 May 2016
First published
13 May 2016

Chem. Commun., 2016,52, 7505-7508

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Simple and sensitive microbial pathogen detection using a label-free DNA amplification assay

Y. Sun, C. Zhao, Z. Yan, J. Ren and X. Qu, Chem. Commun., 2016, 52, 7505 DOI: 10.1039/C6CC02672A

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