Two-armed lanthanide-binding tags induce significant, long-range paramagnetic effects in the NMR spectra of attached proteins. An enantiomeric pair of rigid, two-armed, cyclen-based tags are reported that produce markedly different effects from the same tagging site, allowing for the measurement of complementary paramagnetic restraints for structural studies.
M. D. Lee, M. L. Dennis, J. D. Swarbrick and B. Graham, Chem. Commun., 2016, 52, 7954 DOI: 10.1039/C6CC02325H
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