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Issue 34, 2016
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Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

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Abstract

Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.

Graphical abstract: Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

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Publication details

The article was received on 16 Feb 2016, accepted on 21 Mar 2016 and first published on 23 Mar 2016


Article type: Communication
DOI: 10.1039/C6CC01426G
Citation: Chem. Commun., 2016,52, 5828-5831
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    Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

    H. Park, J. Y. Koo, Y. V. V. Srikanth, S. Oh, J. Lee, J. Park and S. B. Park, Chem. Commun., 2016, 52, 5828
    DOI: 10.1039/C6CC01426G

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