Jump to main content
Jump to site search

Issue 31, 2016
Previous Article Next Article

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Author affiliations

Abstract

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Graphical abstract: Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Jan 2016, accepted on 11 Mar 2016 and first published on 11 Mar 2016


Article type: Communication
DOI: 10.1039/C6CC00223D
Citation: Chem. Commun., 2016,52, 5467-5469
  •   Request permissions

    Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

    F. Caschera, J. W. Lee, K. K. Y. Ho, A. P. Liu and M. C. Jewett, Chem. Commun., 2016, 52, 5467
    DOI: 10.1039/C6CC00223D

Search articles by author

Spotlight

Advertisements