Issue 26, 2016

Recent advances in transcription factor assays in vitro

Abstract

Transcription factors (TFs) play central roles in the regulation of gene expression through binding to specific DNA sequences, and may influence multiple transcription-associated cellular processes including cell development, differentiation, and growth. Alterations in TF levels may lead to a variety of human diseases. Consequently, rapid and sensitive detection of TFs is crucial to both biological research and clinical diagnostics. However, conventional methods for TF assays are usually laborious and time-consuming with poor sensitivity, and sometimes involve the radioactive materials. To overcome these limitations, some new approaches have been developed with a low detection limit, high specificity, high throughput, and low cost. In this paper, we review the recent advances in TF assays and highlight the emerging trends as well.

Graphical abstract: Recent advances in transcription factor assays in vitro

Article information

Article type
Feature Article
Submitted
01 Dec 2015
Accepted
18 Feb 2016
First published
18 Feb 2016

Chem. Commun., 2016,52, 4739-4748

Recent advances in transcription factor assays in vitro

Y. Zhang, F. Ma, B. Tang and C. Zhang, Chem. Commun., 2016, 52, 4739 DOI: 10.1039/C5CC09891B

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